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braf rabbit igg  (Proteintech)


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    Proteintech braf rabbit igg
    Proteomic analysis of Tg2576 mice infused with blood from old and young wild type mice. ( A ) Heatmap of quantifiable proteins from the Old Blood and Young Blood groups, showing log fold-change (logFC) in protein expression. ( B ) Venn diagram of differentially expressed proteins in an Old Blood vs. Young Blood groups comparison. ( C ) Canonical pathway analysis of differentially expressed proteins, highlighting enriched signaling pathways with activation z-scores. ( D – F ) Differentially expressed proteins in cAMP-mediated signaling, synaptogenesis signaling, and endocannabinoid neuronal synapse pathways, respectively. Gene names are used. ( G , H ) Representative western blot image and quantitative analysis of expression of CACNA2D2 in brain homogenates. ( I , J ) Representative western blot image and quantitative analysis of expression of <t>BRAF</t> in brain homogenates. ( K , L ) Representative western blot image and quantitative analysis of expression of Syngap1 in brain homogenates. ( M , N ) Representative western blot image and quantitative analysis of expression of MAPK9 in brain homogenates. ( O , P ) Representative western blot image and quantitative analysis of expression of GRK2 in brain homogenates. N = 3/group for proteomic analysis, and n = 4/group for protein validation (random mix of males and females; young donor group: 1–2M/2F; old donor group: 1–2M/2F). Data are expressed as mean ± SEM. Data in ( H ), ( J ), ( L ), ( N ), and ( P ) were analyzed using Student’s t -test. * p < 0.05.
    Braf Rabbit Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Infusion of blood from young and old mice modulates amyloid pathology"

    Article Title: Infusion of blood from young and old mice modulates amyloid pathology

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.206319

    Proteomic analysis of Tg2576 mice infused with blood from old and young wild type mice. ( A ) Heatmap of quantifiable proteins from the Old Blood and Young Blood groups, showing log fold-change (logFC) in protein expression. ( B ) Venn diagram of differentially expressed proteins in an Old Blood vs. Young Blood groups comparison. ( C ) Canonical pathway analysis of differentially expressed proteins, highlighting enriched signaling pathways with activation z-scores. ( D – F ) Differentially expressed proteins in cAMP-mediated signaling, synaptogenesis signaling, and endocannabinoid neuronal synapse pathways, respectively. Gene names are used. ( G , H ) Representative western blot image and quantitative analysis of expression of CACNA2D2 in brain homogenates. ( I , J ) Representative western blot image and quantitative analysis of expression of BRAF in brain homogenates. ( K , L ) Representative western blot image and quantitative analysis of expression of Syngap1 in brain homogenates. ( M , N ) Representative western blot image and quantitative analysis of expression of MAPK9 in brain homogenates. ( O , P ) Representative western blot image and quantitative analysis of expression of GRK2 in brain homogenates. N = 3/group for proteomic analysis, and n = 4/group for protein validation (random mix of males and females; young donor group: 1–2M/2F; old donor group: 1–2M/2F). Data are expressed as mean ± SEM. Data in ( H ), ( J ), ( L ), ( N ), and ( P ) were analyzed using Student’s t -test. * p < 0.05.
    Figure Legend Snippet: Proteomic analysis of Tg2576 mice infused with blood from old and young wild type mice. ( A ) Heatmap of quantifiable proteins from the Old Blood and Young Blood groups, showing log fold-change (logFC) in protein expression. ( B ) Venn diagram of differentially expressed proteins in an Old Blood vs. Young Blood groups comparison. ( C ) Canonical pathway analysis of differentially expressed proteins, highlighting enriched signaling pathways with activation z-scores. ( D – F ) Differentially expressed proteins in cAMP-mediated signaling, synaptogenesis signaling, and endocannabinoid neuronal synapse pathways, respectively. Gene names are used. ( G , H ) Representative western blot image and quantitative analysis of expression of CACNA2D2 in brain homogenates. ( I , J ) Representative western blot image and quantitative analysis of expression of BRAF in brain homogenates. ( K , L ) Representative western blot image and quantitative analysis of expression of Syngap1 in brain homogenates. ( M , N ) Representative western blot image and quantitative analysis of expression of MAPK9 in brain homogenates. ( O , P ) Representative western blot image and quantitative analysis of expression of GRK2 in brain homogenates. N = 3/group for proteomic analysis, and n = 4/group for protein validation (random mix of males and females; young donor group: 1–2M/2F; old donor group: 1–2M/2F). Data are expressed as mean ± SEM. Data in ( H ), ( J ), ( L ), ( N ), and ( P ) were analyzed using Student’s t -test. * p < 0.05.

    Techniques Used: Expressing, Comparison, Protein-Protein interactions, Activation Assay, Western Blot, Biomarker Discovery



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    Proteomic analysis of Tg2576 mice infused with blood from old and young wild type mice. ( A ) Heatmap of quantifiable proteins from the Old Blood and Young Blood groups, showing log fold-change (logFC) in protein expression. ( B ) Venn diagram of differentially expressed proteins in an Old Blood vs. Young Blood groups comparison. ( C ) Canonical pathway analysis of differentially expressed proteins, highlighting enriched signaling pathways with activation z-scores. ( D – F ) Differentially expressed proteins in cAMP-mediated signaling, synaptogenesis signaling, and endocannabinoid neuronal synapse pathways, respectively. Gene names are used. ( G , H ) Representative western blot image and quantitative analysis of expression of CACNA2D2 in brain homogenates. ( I , J ) Representative western blot image and quantitative analysis of expression of <t>BRAF</t> in brain homogenates. ( K , L ) Representative western blot image and quantitative analysis of expression of Syngap1 in brain homogenates. ( M , N ) Representative western blot image and quantitative analysis of expression of MAPK9 in brain homogenates. ( O , P ) Representative western blot image and quantitative analysis of expression of GRK2 in brain homogenates. N = 3/group for proteomic analysis, and n = 4/group for protein validation (random mix of males and females; young donor group: 1–2M/2F; old donor group: 1–2M/2F). Data are expressed as mean ± SEM. Data in ( H ), ( J ), ( L ), ( N ), and ( P ) were analyzed using Student’s t -test. * p < 0.05.
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    Proteomic analysis of Tg2576 mice infused with blood from old and young wild type mice. ( A ) Heatmap of quantifiable proteins from the Old Blood and Young Blood groups, showing log fold-change (logFC) in protein expression. ( B ) Venn diagram of differentially expressed proteins in an Old Blood vs. Young Blood groups comparison. ( C ) Canonical pathway analysis of differentially expressed proteins, highlighting enriched signaling pathways with activation z-scores. ( D – F ) Differentially expressed proteins in cAMP-mediated signaling, synaptogenesis signaling, and endocannabinoid neuronal synapse pathways, respectively. Gene names are used. ( G , H ) Representative western blot image and quantitative analysis of expression of CACNA2D2 in brain homogenates. ( I , J ) Representative western blot image and quantitative analysis of expression of <t>BRAF</t> in brain homogenates. ( K , L ) Representative western blot image and quantitative analysis of expression of Syngap1 in brain homogenates. ( M , N ) Representative western blot image and quantitative analysis of expression of MAPK9 in brain homogenates. ( O , P ) Representative western blot image and quantitative analysis of expression of GRK2 in brain homogenates. N = 3/group for proteomic analysis, and n = 4/group for protein validation (random mix of males and females; young donor group: 1–2M/2F; old donor group: 1–2M/2F). Data are expressed as mean ± SEM. Data in ( H ), ( J ), ( L ), ( N ), and ( P ) were analyzed using Student’s t -test. * p < 0.05.
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    Proteomic analysis of Tg2576 mice infused with blood from old and young wild type mice. ( A ) Heatmap of quantifiable proteins from the Old Blood and Young Blood groups, showing log fold-change (logFC) in protein expression. ( B ) Venn diagram of differentially expressed proteins in an Old Blood vs. Young Blood groups comparison. ( C ) Canonical pathway analysis of differentially expressed proteins, highlighting enriched signaling pathways with activation z-scores. ( D – F ) Differentially expressed proteins in cAMP-mediated signaling, synaptogenesis signaling, and endocannabinoid neuronal synapse pathways, respectively. Gene names are used. ( G , H ) Representative western blot image and quantitative analysis of expression of CACNA2D2 in brain homogenates. ( I , J ) Representative western blot image and quantitative analysis of expression of <t>BRAF</t> in brain homogenates. ( K , L ) Representative western blot image and quantitative analysis of expression of Syngap1 in brain homogenates. ( M , N ) Representative western blot image and quantitative analysis of expression of MAPK9 in brain homogenates. ( O , P ) Representative western blot image and quantitative analysis of expression of GRK2 in brain homogenates. N = 3/group for proteomic analysis, and n = 4/group for protein validation (random mix of males and females; young donor group: 1–2M/2F; old donor group: 1–2M/2F). Data are expressed as mean ± SEM. Data in ( H ), ( J ), ( L ), ( N ), and ( P ) were analyzed using Student’s t -test. * p < 0.05.
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    Proteomic analysis of Tg2576 mice infused with blood from old and young wild type mice. ( A ) Heatmap of quantifiable proteins from the Old Blood and Young Blood groups, showing log fold-change (logFC) in protein expression. ( B ) Venn diagram of differentially expressed proteins in an Old Blood vs. Young Blood groups comparison. ( C ) Canonical pathway analysis of differentially expressed proteins, highlighting enriched signaling pathways with activation z-scores. ( D – F ) Differentially expressed proteins in cAMP-mediated signaling, synaptogenesis signaling, and endocannabinoid neuronal synapse pathways, respectively. Gene names are used. ( G , H ) Representative western blot image and quantitative analysis of expression of CACNA2D2 in brain homogenates. ( I , J ) Representative western blot image and quantitative analysis of expression of BRAF in brain homogenates. ( K , L ) Representative western blot image and quantitative analysis of expression of Syngap1 in brain homogenates. ( M , N ) Representative western blot image and quantitative analysis of expression of MAPK9 in brain homogenates. ( O , P ) Representative western blot image and quantitative analysis of expression of GRK2 in brain homogenates. N = 3/group for proteomic analysis, and n = 4/group for protein validation (random mix of males and females; young donor group: 1–2M/2F; old donor group: 1–2M/2F). Data are expressed as mean ± SEM. Data in ( H ), ( J ), ( L ), ( N ), and ( P ) were analyzed using Student’s t -test. * p < 0.05.

    Journal: Aging (Albany NY)

    Article Title: Infusion of blood from young and old mice modulates amyloid pathology

    doi: 10.18632/aging.206319

    Figure Lengend Snippet: Proteomic analysis of Tg2576 mice infused with blood from old and young wild type mice. ( A ) Heatmap of quantifiable proteins from the Old Blood and Young Blood groups, showing log fold-change (logFC) in protein expression. ( B ) Venn diagram of differentially expressed proteins in an Old Blood vs. Young Blood groups comparison. ( C ) Canonical pathway analysis of differentially expressed proteins, highlighting enriched signaling pathways with activation z-scores. ( D – F ) Differentially expressed proteins in cAMP-mediated signaling, synaptogenesis signaling, and endocannabinoid neuronal synapse pathways, respectively. Gene names are used. ( G , H ) Representative western blot image and quantitative analysis of expression of CACNA2D2 in brain homogenates. ( I , J ) Representative western blot image and quantitative analysis of expression of BRAF in brain homogenates. ( K , L ) Representative western blot image and quantitative analysis of expression of Syngap1 in brain homogenates. ( M , N ) Representative western blot image and quantitative analysis of expression of MAPK9 in brain homogenates. ( O , P ) Representative western blot image and quantitative analysis of expression of GRK2 in brain homogenates. N = 3/group for proteomic analysis, and n = 4/group for protein validation (random mix of males and females; young donor group: 1–2M/2F; old donor group: 1–2M/2F). Data are expressed as mean ± SEM. Data in ( H ), ( J ), ( L ), ( N ), and ( P ) were analyzed using Student’s t -test. * p < 0.05.

    Article Snippet: The membranes were incubated with the following primary antibodies overnight at 4°C in agitation: 6E10 mouse/IgG1 (Biolegend, San Diego, CA, USA) (1/500), α2δ2 ( Cacna2d2 ) rabbit/IgG (Abcam, Fremont, CA, USA) (1:1000), SynGAP1 rabbit/IgG (Abcam, Fremont, CA, USA), BRAF rabbit/IgG (Proteintech, Rosemont, IL, USA), JNK2 (MAPK9) mouse/IgG (Origene, Rockville, MD, USA), GRK2 mouse/IgG1 (Invitrogen, Carlsbad, CA, USA), and β-Actin mouse/IgG2b (Cellsignal, Danvers, MA, USA).

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